产品名称

Anti-alpha Tubulin抗体[DM1A] - Loading Control

参阅全部 alpha Tubulin 一抗

描述

小鼠单克隆抗体[DM1A] to alpha Tubulin - Loading Control

宿主

Mouse

经测试应用

适用于: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details

种属反应性

与反应: Mouse, Rat, Human

预测可用于: Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Xenopus laevis, Gerbil, African green monkey

免疫原

Full length native protein (purified) corresponding to Chicken alpha Tubulin.

阳性对照

WB: HeLa, HEK293, HepG2, Caco2, NIH3T3, PC12 cell lysates. Flow Cyt (Intra): methanol fixed/Tween permeabilised HeLa cells. ICC/IF: Caco-2, NIH3T3, and SV40LT-SMC cells. IHC-P: Human colon and rat colon tissues.

常规说明

This antibody clone [DM1A] is manufactured by Abcam.

Excellent as a protein loading control antibody. DM1A causes the 10 nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. See references for further information on the above.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As