10306-1-AP targets B23/NPM1 in WB, IP, IF, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, rat samples.

Tested ApplicationsIF/ICC, IHC, IP, WB, ELISA Application Description

Cited ApplicationsWB, IP, IF, IHC, CoIP, ChIP

Tested ReactivityHuman, Rat

Cited ReactivityHuman, Mouse, Rat, Pig

ImmunogenB23/NPM1 fusion protein Ag0286 种属同源性预测

Host / IsotypeRabbit / IgG

ClassPolyclonal

TypeAntibody

Full Namenucleophosmin (nucleolar phosphoprotein B23, numatrin)

SynonymsB23, B23/NPM1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

Calculated Molecular Weight33 kDa

Observed Molecular Weight35-40 kDa

GenBank Accession NumberBC002398

Gene SymbolNPM1

Gene ID (NCBI)4869

RRIDAB_2155163

ConjugateUnconjugated

FormLiquid

Purification MethodAntigen affinity purification

UNIPROT IDP06748

Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.