产品名称

Iron Assay试剂盒(Colorimetric)

参阅全部 Iron 试剂盒

检测方法

Colorimetric

样品类型

Urine, Serum, Other biological fluids

检测类型

Quantitative

灵敏度

> 8 µM

范围

8 µM - 400 µM

检测时间

1h 30m

产品概述

Iron Assay Kit ab83366 provides a simple convenient means of measuring ferrous (Fe2+) and/or ferric (Fe3+) iron in biological samples.

Ferric carrier protein will dissociate ferric into solution in the presence of the acid assay buffer. If the assay buffer has a neutral pH, iron will bind tightly to the iron carrier protein. However, under acidic conditions (pH less than 5.5), iron no longer has a binding affinity for the carrier protein and will be dissociated and released into the solution as iron/ferric ions, whose concentration you can measure using this kit. The Iron Assay Buffer in this kit has an acidic pH that enables this release of iron/ferric ions into the solution.

Free ferrous iron (Fe2+) reacts with Iron Probe to produce a stable colored complex with absorbance at 593 nm. Ferric iron (Fe3+) can be reduced to form Fe2+ enabling the measurement of total iron (Fe2+ and Fe3+). The level of ferric iron (Fe3+) is calculated by subtracting ferrous iron from total iron.

A specific chelate chemical is included in the buffer to block copper ion (Cu2+) interference.

The kit measures iron in the linear range of 0.4 to 10 nmol or 8 µM to 200 µM.

Iron assay protocol summary:

- add samples and standards to wells

- for Fe2+ assay add assay buffer only, for total iron (Fe2+ and Fe3+) add iron reducer

- incubate for 30 min at 37ºC

- add Iron Probe and incubate for 60 min at 37ºC

- analyze with microplate reader

说明

This product is manufactured by BioVision, an Abcam company and was previously called K390 Iron Colorimetric Assay Kit. K390-100 is the same size as the 100 test size of ab83366.

平台

Microplate reader